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Scientists reveal DNA-enzyme interaction with first ever real time footage

14 September 2007

For the first time scientists have been able to film, in real-time, the nanoscale interaction of an enzyme and a DNA strand from an attacking virus. Researchers from the University of Cambridge have used a revolutionary Scanning Atomic Force Microscope in Japan to produce amazing footage of a protective enzyme unravelling the DNA of a virus trying to infect a bacterial host.

The research, funded by the Biotechnology and Biological Sciences Research Council (BBSRC), will improve our understanding of how enzymes interact with DNA at a nanoscale and also has marked implications for scientists studying DNA repair, and could help in the search for cancer treatments.

Working with researchers in Edinburgh, Japan and India, the Cambridge team used a state-of-the-art microscope, only three of which exist in the world, and a technique known as fast-scan atomic force microscopy (AFM). Before now, scientists could only make assumptions as to how proteins and DNA interact based on indirect evidence but this new window on a fundamental biological process gives them the opportunity to view how the interaction actually occurs.

Dr Robert Henderson, who led the Cambridge research, explains: “This is the first time that such a process has been seen in real time. To be able see these nano-mechanisms as they are really happening is incredibly exciting. We can actually see the enzyme ‘threading’ through a loop in the virus’s DNA in order to lock on to and break it, a process known as DNA cleavage.

“The microscope and new techniques give us a clear view of the molecular interactions between proteins and DNA that we could only previously interpret indirectly. The indirect methods require scientists to make assumptions to interpret their data, and video footage like this can help to provide a more direct understanding of what is really happening.

“Standard technology for filming on this scale can only produce one image frame every 8 minutes. However, our new work allows one frame per 500 – or fewer, milliseconds.”

The footage shows a bacterial type III restriction enzyme attaching itself to the DNA of a virus, in order to break the DNA before the virus has the chance to infect the bacterium. However, this could also provide a model for understanding how other enzymes and DNA, in any type of organism, including humans, interact.

“This helps us understand how enzymes recognise which bit of a DNA strand to latch onto, which is important in understanding how proteins repair damaged DNA. In the long term, this could help in the search for cancer treatments, as cancer sometimes occurs where DNA is damaged but enzymes do not behave correctly in order to repair it.”

Steve Visscher, interim BBSRC Chief Executive, said: “BBSRC strongly supports the development of new tools and resources and this study clearly highlights the significance of cutting-edge technologies to bioscience research. It is essential that bioscientists can draw upon technologies from the physical and engineering sciences to improve their understanding of biological processes.”

ENDS

Notes to editors

This project was a collaboration between the University of Cambridge’s Department of Pharmacology, researchers at Kyoto University (led by Professor Kunio Takeyasu), University of Edinburgh (Dr David Dryden) and the Indian Institute of Science (Dr Desirazu Rao).  

It was supported in the UK by BBSRC and in Japan by the Ministry of Education, Culture, Sports, Science and Technology and the Ministry of Economy, Trade and Industry.

Travel support for the project came from BBSRC’s International Scientific Interchange Scheme.

This film was originally published online on July 23, 2007 in the journal The Proceedings of the National Academy of Sciences (PNAS), 10.1073/pnas.0700483104

About BBSRC

The Biotechnology and Biological Sciences Research Council (BBSRC) is the UK funding agency for research in the life sciences. Sponsored by Government, BBSRC annually invests around £380 million in a wide range of research that makes a significant contribution to the quality of life for UK citizens and supports a number of important industrial stakeholders including the agriculture, food, chemical, healthcare and pharmaceutical sectors. http://www.bbsrc.ac.uk

Video

Nanoscale interaction of an enzyme and a DNA strand from an attacking virus, in real-time.

  First ever real time film of DNA-enzyme interaction

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This film was originally published online on July 23, 2007 in the journal The Proceedings of the National Academy of Sciences (PNAS), 10.1073/pnas.0700483104

© 2007, National Academy of Sciences, U.S.A.
Permission to republish the video must be requested from the National Academy of Sciences.

External contact

Dr Robert Henderson, University of Cambridge

tel: 01223 334053

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